Reduced DIDS-sensitive chloride conductance in Ae1−/− mouse erythrocytes
نویسندگان
چکیده
منابع مشابه
Electrodiffusion, Barrier, and Gating Analysis of DIDS-insensitive Chloride Conductance in Human Red Blood Cells Treated with Valinomycin or Gramicidin
Current-voltage curves for DIDS-insensitive Cl- conductance have been determined in human red blood cells from five donors. Currents were estimated from the rate of cell shrinkage using flow cytometry and differential laser light scattering. Membrane potentials were estimated from the extracellular pH of unbuffered suspensions using the proton ionophore FCCP. The width of the Gaussian distribut...
متن کاملVoltage dependence of DIDS-insensitive chloride conductance in human red blood cells treated with valinomycin or gramicidin
Net K and Cl effluxes induced by valinomycin or by gramicidin have been determined directly at varied external K, denoted by [K]o, in the presence and absence of the anion transport inhibitors DIDS (4,4'-diiso-thiocyano-2,2'-disulfonic acid stilbene), and its less potent analogue SITS (4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid). The results confirm that pretreatment with 10 micro...
متن کاملRegulation of cell proliferation by intermediate-conductance Ca -activated potassium and volume-sensitive chloride channels in mouse mesenchymal stem cells
Tao R, Lau CP, Tse HF, Li GR. Regulation of cell proliferation by intermediate-conductance Ca -activated potassium and volumesensitive chloride channels in mouse mesenchymal stem cells. Am J Physiol Cell Physiol 295: C1409–C1416, 2008. First published September 24, 2008; doi:10.1152/ajpcell.00268.2008.—Bone marrow mesenchymal stem cells (MSCs) are a promising cell source for regenerative medici...
متن کاملRegulation of cell proliferation by intermediate-conductance Ca2+-activated potassium and volume-sensitive chloride channels in mouse mesenchymal stem cells.
Bone marrow mesenchymal stem cells (MSCs) are a promising cell source for regenerative medicine; however, their cellular physiology is not fully understood. The present study aimed at exploring the potential roles of the two dominant functional ion channels, intermediate-conductance Ca(2+)-activated potassium (IK(Ca)) and volume-sensitive chloride (I(Cl.vol)) channels, in regulating proliferati...
متن کاملExtracellular adenosine modulates a volume-sensitive-like chloride conductance in immortalized rabbit DC1 cells.
Cl(-) currents induced by cell swelling were characterized in an immortalized cell line (DC1) derived from rabbit distal bright convoluted tubule by the whole cell patch-clamp techniques and by (125)I(-) efflux experiments. Exposure of cells to a hypotonic shock induced outwardly rectifying Cl(-) currents that could be blocked by 0.1 mM 5-nitro-2-(3-phenylpropyl-amino)benzoic acid, 1 mM DIDS, a...
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ژورنال
عنوان ژورنال: Blood Cells, Molecules, and Diseases
سال: 2008
ISSN: 1079-9796
DOI: 10.1016/j.bcmd.2008.01.002